Ultrabright Fluorescent Polymeric Nanoparticles with a Stealth Pluronic Shell for Live Tracking in the Mouse Brain. Igor Khalin, Doriane Heimburger, Nina Melnychuk, Mayeul Collot, Bernhard Groschup, Farida Hellal, Andreas Reisch, Nikolaus Plesnila, Andrey S.
ACS Applied Materials & Interfaces 2021, 13 Construction of Enhanced Photostability Anthraquinone-Type Nanovesicles Based on a Novel Two-Step Supramolecular Assembly Strategy and Their Application on Multiband Laser-Responsive Composites. Linlin Su, Lan Shu, Binbin Shi, Yixiao Hang, Jin Huang.This article is cited by 12 publications. The obtained dye-loaded ZI polymer NPs open the route to intracellular single-particle tracking and biosensing applications. In addition, single-particle tracking showed up to four times faster diffusion of ZI NPs in the cytosol compared to PEGylated NPs. The combination of the very small size with the nonfouling nature of these particles enables spreading of ZI polymer NPs in the whole cytosol after their microinjection into living cells. We found that 10 mol % ZI groups in the polymer yield NPs of less than 15 nm that are stable in physiological salt conditions and practically resistant to protein adsorption, as suggested by fluorescence correlation spectroscopy. Dye-loaded polymer NPs are assembled through nanoprecipitation of the copolymer together with the salt of a rhodamine B derivative and a bulky hydrophobic counterion to achieve high particle brightness. Here, we propose a one-step process for preparing ultrasmall and bright stealth NPs based on a zwitterionic (ZI) methacrylate-based copolymer. Combining these properties requires implementing the stealth behavior through the thinnest possible hydrophilic shell. The solution is to develop very small, bright, and noninteracting (stealth) NPs. Intracellular applications of fluorescent nanoparticles (NPs) as probes and labels are currently limited by significant molecular crowding and the high level of complexity encountered inside living cells.